Tandem Mass Spectrometry Basics

Tandem mass spectroscopy basics 1.

Tandem mass spectrometry basics. Many mass spectrometers use two or more mass analyzers for tandem mass spectrometry ms ms. An overview presented by michelle l. Monoisotopic mass the sum of the exact masses of the most abundant stable isotope of the atoms that make up a molecule e g. One such method involves colliding the ions with a stream of inert gas which is known as collision induced dissociation cid or higher energy collision dissociation hcd.

Coupling a quadrupole and time of flight resulted in the production of high resolution mass spectrometers i e q tof. Tandem mass spectrometry ms ms tandem mass spectrometry ms ms offers additional information about specific ions. Tandem mass spectrometry ms ms tandem ms or ms ms msn is a technique to break down selected ions precursor ions into fragments product ions. Mass spectrometry ul li mass spectrometry is the study of systems causing the formation of gaseous ions with or without fragmentation which are then characterised by their mass to charge ratios m z and relative abundances li ul 3.

Average mass the sum of the atomic weights of all the atoms in a molecule e g. C 12 0111 h 1 0079 o 15 9994. Mcluckey purdue university on june 14 2014. A common use of tandem ms is the analysis of biomolecules such as proteins and peptides.

The fragments then reveal aspects of the chemical structure of the precursor ion. Tandem mass spectrometry ms ms as the name indicates is the result of performing two or more sequential separations of ions usually coupling two or more mass analyzers. C 12 0000 h 1 00783 o 15 9949 nominal mass. The detector records the charge induced or the current produced when an ion passes by or hits a surface.

The basics of mass spectrometry series. In this approach distinct ions of interest are in a quadrupole filter based on their m z during the first round of ms and are fragmented by a number of different dissociation methods. Ifa york university on november 8 2013. Thank to tandem mass spectrometry it is easily used to elucidate the structure and chemical properties of molecules and complex.

Tandem mass spectrometry or ms ms presented by scott a. Implmentation of tms at bhh 2. Tandem mass spectrometry begins as a typical mass spec instrument. The following scheme explains how tandem ms works.

With an ion source which converts the sample into ions and a mass analyzer which separates the ions based on their mass to charge ratio. Mass spectrometry is a powerful analytical technique used to identify chemical substances within very few sample.